Protein Expression &
Antibody Development Platform

From antigen design to fully characterized antibody — Sekbio's mammalian expression systems and end-to-end antibody development services accelerate your IVD and biopharmaceutical programs.

7-Day CHO Delivery Up to 2.4 g/L Yield AI-Optimized Design 1,800+ Expressions Completed ISO 9001:2015 QC
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800+
Successful Expression Projects
1,800+
Proteins Expressed (HEK293)
7
Days — CHO Express Delivery
2.4 g/L
Peak CHO Expression Yield

CHO Expression Platform

Sekbio's proprietary CHO system represents the pinnacle of mammalian cell expression technology — delivering unprecedented speed and yield for protein production, from milligram screening to >100 L manufacturing.

CHO High Throughput
Screening of 1,000+ constructs for optimal expression conditions
CHO Express
10 mg protein delivery in minimal turnaround — ideal for urgent timelines
CHO High Performance
Large-scale production for preclinical studies and IVD raw material supply
CHO Stable Cell Lines
Clonal cell line development for consistent, long-term production

Core Advantages

  • 7-Day Expression: Rapid protein delivery for urgent research needs
  • High-Yield Performance: Achieves up to 2.4 g/L protein expression
  • AI-Optimized Design: Machine learning-driven construct optimization
  • Scalable Production: From 1 mL screening to >100 L manufacturing
  • Comprehensive QC: >90% purity with full analytical characterization
Purity: >90% by SEC-HPLC Endotoxin: <1.0 EU/mg Aggregation: <5% by SEC Scale: 1 mL → 100+ L
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Supported Protein Types

  • Monoclonal antibodies (IgG, IgM, IgA)
  • Bispecific and multispecific antibodies
  • Antibody fragments — Fab, scFv, VHH (nanobody)
  • Fusion proteins and Fc fusion constructs
  • Cytokines and growth factors
  • Enzymes and therapeutic proteins
  • Recombinant antigens for IVD raw materials

HEK293 Expression Systems

Complementing our CHO platform, Sekbio offers comprehensive HEK293 transient and stable expression services — with 1,800+ successful protein expressions completed to date.

Available Cell Lines

  • HD293F — high-density suspension culture
  • Expi293F — enhanced transient expression
  • Expi293 GnTI⁻ — afucosylated glycan engineering
Scale: 40 mL – 200 L bioreactor Glycoengineering: Controlled glycosylation Track Record: 1,800+ proteins

Why Choose HEK293?

HEK293 systems produce proteins with human-like post-translational modifications and glycosylation patterns — critical for proteins where glycan structure affects function, immunogenicity, or half-life.

Key Applications

  • Antibodies requiring native glycosylation
  • Recombinant antigens for diagnostic assay development
  • Glycoengineered antibodies (afucosylated for ADCC)
  • Virus-like particles (VLPs) and complex assemblies
  • Rapid feasibility and expression screening
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Monoclonal Antibody Development

End-to-end mouse monoclonal antibody services — from antigen design and custom immunization to fully characterized antibody production. Lead candidates delivered in 8–12 weeks.

1
Antigen Preparation & Immunization
  • Recombinant protein expression and purification
  • Peptide synthesis and carrier conjugation
  • Custom immunization protocols (4–8 weeks)
  • Serum titer monitoring and optimization
2
Hybridoma Generation & Screening
  • Spleen cell fusion and hybridoma selection
  • High-throughput ELISA screening
  • Single-cell cloning by limiting dilution
  • Expansion and cryopreservation of positive clones
3
Antibody Characterization
  • Binding Analysis: ELISA, Western blot, flow cytometry
  • Affinity Measurement: SPR/BLI kinetics (KD determination)
  • Specificity: Cross-reactivity and epitope mapping
  • Functional Testing: Neutralization, blocking, effector function
4
Production & Purification
  • Small-scale (mg) to large-scale (g) production
  • Protein A/G affinity chromatography
  • Buffer exchange and formulation optimization
  • Full QC documentation package
>90%
Hybridoma Success Rate
20–50
Specific Clones per Fusion
nM–pM
Achievable Affinity Range
8–12 wk
To Lead Candidates
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Specialized Capabilities

Beyond standard expression and hybridoma services, Sekbio offers advanced engineering and screening technologies to solve the most challenging antibody development problems.

AI-Assisted Antibody Design

  • Computational antibody-antigen interaction prediction
  • Optimized library design with AI-guided diversity
  • In silico affinity maturation
  • Developability assessment for manufacturability

High-Throughput Screening

  • Beacon® optofluidic system for single-cell analysis
  • Next-generation sequencing of antibody repertoires
  • Microfluidic screening for rare clones
  • Automated colony picking and expansion

Antibody Engineering

  • Humanization: CDR grafting & framework optimization
  • Affinity Maturation: Library construction & screening
  • Format Conversion: IgG → Fab, scFv, or VHH
  • Bispecific Engineering: T-cell engagers & dual-targeting

Technical Specifications & Quality Metrics

Mammalian Expression Quality

ParameterSpecification
Purity>90% by SEC-HPLC and SDS-PAGE
Endotoxin<1.0 EU/mg
Aggregation<5% by SEC-HPLC
SterilityUSP <71> compliant
ConcentrationVerified by A280 and BCA
Peak Yield (CHO)Up to 2.4 g/L
Scale Range1 mL – >100 L
Quality StandardISO 9001:2015 certified

Antibody Development Success Rates

MilestonePerformance
Hybridoma Generation>90% success rate
Positive Clone IdentificationTypically 20–50 clones / fusion
Achievable AffinitynM to pM range (SPR/BLI confirmed)
Project Completion8–12 weeks to lead candidates
GMP ComplianceGMP-compliant facilities available
DocumentationFull regulatory submission package
Projects Completed800+ expression projects
Global Clients200+ clients worldwide

Ready to Accelerate Your Protein & Antibody Programs?

Our PhD-level scientists are ready to discuss your project requirements. Get a technical consultation and custom quote within 24 hours.

Request a Quote Email info@sekbio.com