Luteinizing Hormone Monoclonal Antibody for Ovulation Detection, Fertility Assessment & PCOS Diagnosis
Luteinizing Hormone (LH) is a 30 kDa glycoprotein gonadotropin produced by the anterior pituitary gland, sharing an alpha subunit with FSH, TSH, and hCG but with a unique LH-beta subunit. In females, the mid-cycle LH surge (a rapid 2–3 fold rise in serum LH over baseline) triggers ovulation 24–36 hours later — making LH the most reliable biochemical predictor of the fertile window. In males, LH stimulates Leydig cell testosterone synthesis.
LH surge detection is the basis of ovulation predictor kits (OPKs) — one of the highest-volume POCT fertility products globally. These urine LFA tests detect the rapid LH rise above a threshold of ~25 IU/L (urine), enabling women to identify their 2-day peak fertility window for timed intercourse or intrauterine insemination (IUI). Serum LH measurement is also central to PCOS diagnosis (elevated LH/FSH ratio >2:1 in ~50% of PCOS patients), hypogonadism assessment, and premature ovarian insufficiency (POI) workup.
For IVD immunoassay development, LH antibodies must target the LH-beta subunit for specificity against FSH, TSH, and hCG. Sekbio's matched pair (LH-W74M + LH-W76M) provides Mouse IgG1 antibodies with distinct epitope recognition for sandwich immunoassay development.
Matched capture and detection antibodies for LH sandwich immunoassay development.
| Catalog No. | Product Name | Isotype | Concentration | Purity | Intended Use | Storage |
|---|---|---|---|---|---|---|
| LH-W74M | Mouse Anti-Human LH mAb (Capture) | Mouse IgG1 | 4.51 mg/mL | >90% SEC-HPLC | Capture — LFA / CLIA / ELISA | +2°C to +8°C |
| LH-W76M | Mouse Anti-Human LH mAb (Detection) | Mouse IgG1 | 4.47 mg/mL | >90% SEC-HPLC | Detection — LFA / CLIA / ELISA | +2°C to +8°C |
Both supplied in 20 mM PB, 150 mM NaCl, pH 7.4. Storage at +2°C to +8°C. MOQ 1 mg. Available as part of Fertility Panel (LH + FSH + AMH + E2 + Progesterone). Contact info@sekbio.com for panel pricing.
LH-W74M and LH-W76M target distinct epitopes on the LH-beta subunit, providing assay specificity against cross-reactive gonadotropins (FSH, TSH) and hCG — which shares 85% beta-subunit amino acid homology with LH. Specificity data available in the product datasheet.
LH antibody pair validated for both urine and serum sample matrices. Ovulation predictor LFA kits use urine LH detection (surge threshold ~25 IU/L); quantitative CLIA assays use serum. Both use the same antibody pair, enabling multi-platform product development from a single procurement source.
Affinity-purified with >90% purity by SEC-HPLC. Mouse IgG1 isotype provides consistent Fc-region characteristics for colloidal gold conjugation in LFA strips and for protein A/G purification in CLIA assay bead-coating workflows.
LH-W74M and LH-W76M are stable at +2°C to +8°C — eliminating the need for −20°C freeze storage for short-term use. Simplifies on-site antibody handling in production environments and reduces cold-chain logistics costs for international OEM supply.
Sekbio supplies LH, FSH, AMH, Estradiol (E2), and Progesterone antibody pairs from a single ISO 13485-certified source. Complete fertility panel procurement streamlines vendor qualification, simplifies regulatory documentation, and enables consistent batch-to-batch performance across all fertility marker reagents.
Manufactured under ISO 13485 QMS with inter-batch CV <10%. Each lot undergoes SEC-HPLC purity, molecular weight, and concentration testing before release. Batch consistency data and CoA documentation support CE IVD, NMPA, and FDA regulatory submissions for LH diagnostic kit manufacturers.
Ovulation predictor kits (OPKs) detect the urine LH surge 24–36 hours before ovulation, identifying the 2-day peak fertile window. The global OPK market exceeds USD 800 million annually. Sekbio's LH antibody pair (LH-W74M + LH-W76M) supports LFA cassette and dipstick development for both consumer home-use OPKs and clinical fertility monitoring POCT devices, with LH-W76M available for colloidal gold conjugation.
Polycystic Ovary Syndrome (PCOS) affects ~10% of reproductive-age women. An elevated LH/FSH ratio >2:1 (measured on day 2–3 of the menstrual cycle) is a hormonal feature of PCOS in approximately 50% of patients. Sekbio's matched LH and FSH antibody pairs enable OEM manufacturers to develop combined LH/FSH ratio CLIA assays, supporting PCOS hormonal profiling panels widely used in reproductive medicine.
Premature ovarian insufficiency (POI) in women under 40 is confirmed by markedly elevated FSH (>25 IU/L) with concurrent elevated LH on two measurements taken 4 weeks apart. Both LH and FSH measurement are required for the diagnostic workup of primary and central hypogonadism. Sekbio's FSH and LH antibody pairs, available from the same supplier, support multi-analyte CLIA panels for reproductive endocrinology.
Digital ovulation monitors (e.g., Clearblue-type devices) quantify urine LH to detect both LH surge peak and the 5-day fertile window using algorithm-based interpretation. Sekbio's LH antibody pair — validated for urine matrix LFA — supports development of both traditional LFA strip-based OPKs and reader-connected digital fertility monitors. ISO 13485 batch documentation facilitates CE IVD registration across EU markets.
LH (Luteinizing Hormone) is a pituitary gonadotropin that triggers ovulation 24–36 hours after its mid-cycle surge in females. Detection of this LH surge via urine POCT tests (ovulation predictor kits) enables women to identify their peak 2-day fertile window for natural conception or timed IUI. Serum LH is also used for PCOS diagnosis (elevated LH/FSH ratio >2:1), hypogonadism workup, and premature ovarian insufficiency assessment.
LH-W74M (Mouse IgG1, capture, 4.51 mg/mL, >90% SEC-HPLC, 20 mM PB pH 7.4, +2–8°C) and LH-W76M (Mouse IgG1, detection, 4.47 mg/mL, >90% SEC-HPLC, 20 mM PB pH 7.4, +2–8°C). Both validated for LFA, CLIA, and ELISA. MOQ 1 mg.
Validated for LFA (urine and serum), CLIA, and ELISA. Supports both POCT ovulation predictor kits (urine LFA) and quantitative clinical laboratory LH measurement for reproductive endocrinology panels.
Typical urine LH OPK assays detect a surge above a threshold of ~25 IU/L, which represents the peak LH concentration associated with imminent ovulation. The LH surge lasts 24–48 hours; ovulation occurs 24–36 hours after surge onset. Some advanced digital OPKs use a semi-quantitative approach to track LH kinetics and identify both rising and peak LH, requiring a wider dynamic range assay.
Yes. Sekbio supplies LH, FSH, AMH, Estradiol (E2), and Progesterone antibody pairs — enabling complete fertility panel procurement from a single ISO 13485-certified source. See also FSH Antibody Pair and AMH Antibody Pair. Contact info@sekbio.com for multi-product panel pricing.
LH-W74M and LH-W76M are stable at +2°C to +8°C (shelf life 3 years from manufacturing date). No −20°C freeze required. Avoid multiple freeze/thaw cycles. Supplied in 20 mM PB, 150 mM NaCl, pH 7.4. Centrifuge before opening.
Request technical datasheet, fertility panel pricing, or discuss OEM supply with our team.