Manufacturer | SEKBIO |
Parameter | AFP |
Linearity | 0.5-1000 ng/ml |
Detection limit | 0.5 ng/ml |
benchmark product | Roche |
Test principle and procedure | AFP: Add 10 μl of sample(The sample is diluted 10 times on the machine) + 30 μl of Ra+ 30 μl of Rd incubation for 15 minutes. Wash with 200 μl of washing solution. Finally, add 100 μl of A + 100 μl of B. |
Sample Concentration(ng/ml) |
RLU | Average | CV | S/N | |
1162.62 | 4,132,851 | 4,047,944 | 4,090,398 | 1.47% | 9130.35 |
644.64 | 2,413,626 | 2,402,215 | 2,407,921 | 0.34% | 5374.82 |
137.78 | 552,114 | 578,005 | 565,060 | 3.24% | 1261.29 |
13.00 | 56,977 | 59,955 | 58,466 | 3.60% | 130.50 |
1.24 | 6,176 | 6,306 | 6,241 | 1.47% | 13.93 |
0.04 | 453 | 443 | 448 | 1.58% | 1.00 |
Sample | RLU | Concentration | Sample | RLU | Concentration |
13 | 62,373 | 13.90 |
644.64 | 2,418,618 | 647.81 |
63,370 | 14.13 | 2,467,418 | 662.12 | ||
59,980 | 13.35 | 2,579,205 | 695.05 | ||
65,255 | 14.57 | 2,384,657 | 637.88 | ||
64,991 | 14.51 | 2,548,509 | 685.99 | ||
63,906 | 14.26 | 2,625,042 | 708.62 | ||
60,879 | 13.56 | 2,441,908 | 654.64 | ||
66,323 | 14.82 | 2,520,405 | 677.70 | ||
60,793 | 13.54 | 2,560,671 | 689.58 | ||
62,603 | 13.95 | 2,453,104 | 657.92 | ||
AVE | 196,623 | 99.27 | AVE | 2,499,954 | 671.73 |
SD | 2110 | 0.49 | SD | 78118 | 22.99 |
CV | 1.07% | 0.49% | CV | 3.12% | 3.42% |
Sample | RLU | Concentration | ||
Ag Diluent | 472 | 508 | 0.004 | 0.011 |
365 | 512 | 0.00 | 0.012 | |
364 | 273 | 0.00 | 0.000 | |
382 | 392 | 0.00 | 0.000 | |
442 | 250 | 0.00 | 0.000 | |
301 | 319 | 0.00 | 0.000 | |
280 | 484 | 0.00 | 0.007 | |
249 | 446 | 0.00 | 0.000 | |
406 | 358 | 0.00 | 0.000 | |
377 | 490 | 0.00 | 0.008 | |
AVE | 916 | 0.09 | ||
SD | 120.35 | 0.05 | ||
AVE+2*SD | 0.19 |
Sample(ng/ml) | Dilution ratio | Concentration value | Average | ||
1303.80 | 1.0 | 1239.37 | 1265.76 | 1274.24 | 1303.799135 |
1173.42 | 0.9 | 1146.01 | 1214.25 | 1094.15 | 1151.25 |
1043.04 | 0.8 | 1032.30 | 1129.58 | 1131.06 | 1097.45 |
912.66 | 0.7 | 959.27 | 1032.66 | 931.43 | 974.29 |
782.28 | 0.6 | 801.08 | 847.83 | 801.65 | 816.81 |
651.90 | 0.5 | 691.66 | 704.87 | 637.77 | 678.01 |
521.52 | 0.4 | 539.84 | 496.45 | 489.92 | 508.68 |
391.14 | 0.3 | 391.37 | 394.63 | 374.61 | 386.86 |
260.76 | 0.2 | 287.37 | 292.72 | 295.31 | 291.80 |
130.38 | 0.1 | 136.52 | 133.42 | 124.08 | 131.33 |
0.02 | 0 | 0.00 | 0.00 | 0.00 | 0.02 |
Reagent | Sample(ng/ml) | Average | Deviation |
2-8° | 1000.00 | 4,619,209 | |
200.00 | 941,520 | ||
40.00 | 174,592 | ||
8.00 | 41,845 | ||
1.00 | 6,160 | ||
0.00 | 657 | ||
37℃ 1d | 1000.00 | 4,541,645 | -1.68% |
200.00 | 951,361 | 1.05% | |
40.00 | 164,523 | -5.77% | |
8.00 | 39,680 | -5.18% | |
1.00 | 5,750 | -6.66% | |
0.00 | 781 | 18.87% | |
37℃ 4d | 1000.00 | 4,672,586 | 1.16% |
200.00 | 946,372 | -0.52% | |
40.00 | 175,190 | 6.48% | |
8.00 | 40,495 | -3.23% | |
1.00 | 5,731 | -0.32% | |
0.00 | 769 | -1.54% | |
37℃ 7d | 1000.00 | 4,334,765 | -6.16% |
200.00 | 900,764 | -4.33% | |
40.00 | 152,647 | -12.57% | |
8.00 | 40,724 | -2.68% | |
1.00 | 5,335 | -13.39% | |
0.00 | 684 | 4.11% |
Reagent | Sample(ng/ml) | Average | Deviation |
2-8℃ | 1000.00 | 4,668,604 | |
200.00 | 877,755 | ||
40.00 | 184,066 | ||
8.00 | 43,028 | ||
1.00 | 7,215 | ||
0.00 | 590 | ||
37℃ 1d | 1000.00 | 4,643,345 | 1.1% |
200.00 | 825,354 | -6.8% | |
40.00 | 196,334 | 5.4% | |
8.00 | 44,191 | 2.8% | |
1.00 | 6,896 | 17.1% | |
0.00 | 567 | -10.3% | |
37℃ 4d | 1000.00 | 4,580,588 | 0.5% |
200.00 | 1,056,764 | -12.3% | |
40.00 | 183,616 | 12.5% | |
8.00 | 37,479 | 5.6% | |
1.00 | 5,374 | 11.9% | |
0.00 | 563 | -13.7% | |
37℃ 7d | 1000.00 | 3,882,314 | -0.84% |
200.00 | 824,447 | 12.24% | |
40.00 | 163,843 | 5.17% | |
8.00 | 37,187 | -10.43% | |
1.00 | 5,881 | -12.77% | |
0.00 | 593 | -14.31% |