S-adenosyl-L-homocysteine hydrolase
CAT. No.: S12-SAHH-4011
Reaction: S-adenosyl-L-homocysteine + H2O → Adenosine + L-homocysteine
Product Description
Appearance: White powder, lyophilized
Source: Microorganism
EC Number: EC 3.3.1.1
CAS Number: 9025-54- 1
Storage temperature: - 20 ℃
Specific activity: ≥2.0 U/mg powder
Unit definition: One unit will hydrolyze one micromole of S-adenosyl-L-homocysteine per min at pH 7.2 at 37 ℃.
Properties | |
| Molecular weight | 44~45 kD |
| Isoelectric point | 5.90 |
| Michaelis constant | 2.3×10-4 M (S-adenosyl-L-homocysteine ) |
| Optimum pH | 6.50 |
| Optimum temperature | 40℃ |
| pH stability | 5.0~ 10.0 (25C, 20hr) |
| Thermal stability | < 45℃ (pH 7.2, 20min) Ag+ |
| Inhibitors | Ag+, Hg2+, EDTA, Cu2+ |
| Effect of various chemicals | ( Table 1 ) |
Table 1
Effect of various chemicals on S-adenosyl-L-homocysteine hydrolase
The enzyme dssolved in 50mM K-phosphate buffer, pH 7.5 ( 2U/ml ) was incubated with each chemical at 37C for 2hr.
| Chemical | Concn. (mM) | Residual activity | Chemical | Concn. (mM) | Residual |
None | - | 100% | EDTA | 5.0 | 0% |
Zn2+ | 2.0 | 50% | NaN3 | 20.0 | 95% |
Cu2+ | 2.0 | 0% | Tween 20 | 0. 10% | 102% |
Fe3+ | 2.0 | 98% | Triton X-100 | 0. 10% | 103% |
Ca2+ | 2.0 | 98% | SDS | 0.05% | 86% |
Mg2+ | 2.0 | 91% | Proclin | 0.05% | 0% |
- Cardiac Markers
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Tumor Marker
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PGII
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G17
- CA50
-
CA125
- CA242
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CA15-3
- CA19-9
- CA72-4
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Pepsinogens I (PGI)
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Human Epididymis 4 (HE4)
- Prostate-Specific Antigen (PSA)
- Squamous Cell Carcinoma (SCC)
- Neuron-Specific Enolase (NSE)
- Cytokeratin 19 Fragment (CYFRA21-1)
- Human Progastrin-releasing Peptide (ProGRP Tumor Marker)
- Protein Induced by Vitamin K Absence or Antagonist-II (PIVKA II Tumor Marker)
- Alpha-fetoprotein(AFP)
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CEA
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Human Chitinase 3-like 1
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PGII
- Inflammatory Marker
- Infectious Disease
- Hormones
- Thyroid Function
- Glucose Metabolism
- Bone Marker
- Others
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Heterophilic Blocking Reagent
- Animal Diagnostics
