S-adenosyl-L-homocysteine hydrolase
CAT. No.: S12-SAHH-4011
Reaction: S-adenosyl-L-homocysteine + H2O → Adenosine + L-homocysteine
Product Description
Appearance: White powder, lyophilized
Source: Microorganism
EC Number: EC 3.3.1.1
CAS Number: 9025-54- 1
Storage temperature: - 20 ℃
Specific activity: ≥2.0 U/mg powder
Unit definition: One unit will hydrolyze one micromole of S-adenosyl-L-homocysteine per min at pH 7.2 at 37 ℃.
Properties | |
Molecular weight | 44~45 kD |
Isoelectric point | 5.90 |
Michaelis constant | 2.3×10-4 M (S-adenosyl-L-homocysteine ) |
Optimum pH | 6.50 |
Optimum temperature | 40℃ |
pH stability | 5.0~ 10.0 (25C, 20hr) |
Thermal stability | < 45℃ (pH 7.2, 20min) Ag+ |
Inhibitors | Ag+, Hg2+, EDTA, Cu2+ |
Effect of various chemicals | ( Table 1 ) |
Table 1
Effect of various chemicals on S-adenosyl-L-homocysteine hydrolase
The enzyme dssolved in 50mM K-phosphate buffer, pH 7.5 ( 2U/ml ) was incubated with each chemical at 37C for 2hr.
Chemical | Concn. (mM) | Residual activity | Chemical | Concn. (mM) | Residual |
None | - | 100% | EDTA | 5.0 | 0% |
Zn2+ | 2.0 | 50% | NaN3 | 20.0 | 95% |
Cu2+ | 2.0 | 0% | Tween 20 | 0. 10% | 102% |
Fe3+ | 2.0 | 98% | Triton X-100 | 0. 10% | 103% |
Ca2+ | 2.0 | 98% | SDS | 0.05% | 86% |
Mg2+ | 2.0 | 91% | Proclin | 0.05% | 0% |
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