Progesterone (Prog)
Progesterone is an endogenous steroid and progestogen sex hormone involved in the menstrual cycle, pregnancy, and embryogenesis of humans and other species. It belongs to a group of steroid hormones called progestogens and is the major progestogen in the body.
Progesterone levels vary during a woman's menstrual cycle. The classes start low, then increase after the ovaries release an egg.
If you become pregnant, progesterone levels will continue to rise as your body gets ready to support a developing baby. If you don't, your progesterone levels will decrease, and your period will begin.
Progesterone levels in a pregnant woman are about ten times higher than those who are not pregnant. In men, the adrenal glands and testes generate progesterone. Men also make progesterone but in much smaller amounts.
The performance data of the Prog rapid Quantitative Test Kit (FIA Test)
Manufacturer | SEKBIO |
Parameter | Progesterone (PROG) Rapid Quantitative Test Kit (FIA Test) |
Linearity | 0.5ng/mL~60ng/mL |
Detection limit | 0.5ng/mL |
Benchmark product | Roche |
1 Limit of detection
Result(C/T) | Concentration | |
0 | 0.46 | |
1 | 0.26445 | 0.50156 |
2 | 0.28688 | 0.43836 |
3 | 0.24489 | 0.51137 |
4 | 0.31987 | 0.42028 |
5 | 0.26411 | 0.44128 |
6 | 0.30127 | 0.42296 |
7 | 0.24441 | 0.52822 |
8 | 0.28998 | 0.44220 |
9 | 0.26857 | 0.44857 |
10 | 0.31376 | 0.48088 |
11 | 0.28120 | 0.41962 |
12 | 0.31124 | 0.52393 |
13 | 0.28228 | 0.44837 |
14 | 0.26100 | 0.39458 |
15 | 0.25996 | 0.51431 |
16 | 0.26714 | 0.43638 |
17 | 0.24321 | 0.46949 |
18 | 0.29104 | 0.51076 |
19 | 0.27968 | 0.41396 |
20 | 0.28350 | 0.50192 |
AVE | 0.27792 | 0.46345 |
SD | 0.02267 | / |
AVE+2*SD | 0.32326 | / |
regression equation | y=0.40332x+0.27792 | |
LoB(ng/mL) | 0.11242 |
2 Linear
Cal | Concentration | Result | AVERAGE |
S1 | 0.46 | 0.42 | 0.46 |
0.50 | |||
0.45 | |||
S2 | 1.78 | 1.57 | 1.67 |
1.75 | |||
1.69 | |||
S3 | 3.34 | 3.05 | 3.40 |
3.51 | |||
3.65 | |||
S4 | 10.76 | 11.75 | 10.68 |
9.35 | |||
10.94 | |||
S5 | 30.27 | 36.27 | 32.26 |
27.57 | |||
32.94 | |||
S6 | 62.32 | 58.23 | 60.26 |
65.90 | |||
56.64 |
3 Accuracy
Cal | concentration | Result | Bias |
A1 | 1.78 | 1.62 | -8.8% |
1.95 | 9.6% | ||
1.86 | 4.4% | ||
A2 | 15.76 | 15.63 | -0.9% |
15.84 | 0.5% | ||
16.48 | 4.6% | ||
A3 | 30.27 | 29.29 | -3.2% |
28.57 | -5.6% | ||
33.17 | 9.6% |
4 Precision
Cal | Concentration | Cal | Concentration | Cal | Concentration |
R1 | 1.56 | R2 | 16.33 | R3 | 32.81 |
1.95 | 13.56 | 32.30 | |||
1.98 | 13.66 | 27.37 | |||
1.57 | 14.60 | 25.93 | |||
1.61 | 16.04 | 26.77 | |||
1.76 | 13.74 | 28.49 | |||
1.53 | 14.23 | 30.94 | |||
1.94 | 14.16 | 30.32 | |||
1.86 | 16.55 | 34.04 | |||
1.88 | 18.05 | 31.55 | |||
AVE | 1.77 | AVE | 15.09 | AVE | 30.05 |
SD | 0.18 | SD | 1.54 | SD | 2.77 |
CV | 10.2% | CV | 10.2% | CV | 9.2% |
5 Method Comparison
Sample | Roche | Sekbio | Sample | Roche | Sekbio |
1 | 30.05 | 30.61 | 31 | 24.17 | 28.12 |
2 | 32.94 | 36.47 | 32 | 7.81 | 8.30 |
3 | 8.42 | 9.80 | 33 | 2.67 | 2.69 |
4 | 48.82 | 46.01 | 34 | 43.60 | 39.92 |
5 | 3.11 | 2.93 | 35 | 27.86 | 30.83 |
6 | 2.18 | 2.13 | 36 | 1.70 | 1.64 |
7 | 0.67 | 0.73 | 37 | 4.77 | 5.82 |
8 | 2.44 | 2.21 | 38 | 2.40 | 2.21 |
9 | 3.31 | 3.42 | 39 | 2.25 | 2.62 |
10 | 10.62 | 9.38 | 40 | 2.68 | 2.94 |
11 | 2.07 | 2.25 | 41 | 5.13 | 4.81 |
12 | 3.62 | 3.11 | 42 | 9.16 | 11.13 |
13 | 4.49 | 4.67 | 43 | 1.09 | 1.12 |
14 | 1.12 | 0.99 | 44 | 44.24 | 39.55 |
15 | 27.63 | 26.30 | 45 | 26.76 | 27.91 |
16 | 24.69 | 27.58 | 46 | 2.33 | 2.61 |
17 | 46.55 | 43.83 | 47 | 1.73 | 2.15 |
18 | 22.46 | 19.65 | 48 | 26.37 | 26.49 |
19 | 7.31 | 7.23 | 49 | 2.23 | 2.39 |
20 | 0.52 | 0.56 | 50 | 1.37 | 1.39 |
21 | 6.75 | 5.41 | 51 | 3.05 | 3.00 |
22 | 20.93 | 21.28 | 52 | 57.55 | 54.59 |
23 | 5.18 | 5.69 | 53 | 2.74 | 2.81 |
24 | 2.44 | 2.64 | 54 | 2.60 | 2.88 |
25 | 1.62 | 1.58 | 55 | 35.71 | 29.40 |
26 | 13.14 | 14.46 | 56 | 7.62 | 7.40 |
27 | 10.40 | 11.68 | 57 | 1.46 | 1.45 |
28 | 17.09 | 15.14 | 58 | 2.06 | 2.16 |
29 | 1.24 | 1.18 | 59 | 1.49 | 1.41 |
30 | 8.75 | 8.87 | 60 | 17.05 | 18.12 |
Progesterone (Prog) Products
Antibody | Application |
Mouse Anti-human Prog mAb | For immunodiagnostic: ELISA, LFA, CLIA |
Progesterone Intro
Progesterone is the main biologically active progesterone secreted by the ovary, and its molecular formula is C21H30O2. Before ovulation, the amount of progesterone hormone produced per day is 2 to 3 mg, mainly from the ovaries.
Progesterone can protect the endometrium of women. After ovulation, it rises to 20~30mg per day, most of which is secreted by the corpus luteum in the ovary. During pregnancy, progesterone hormones can provide support and protection for the early growth and development of the fetus and can play a specific role in calming the uterus. In addition, the relationship between progesterone and estrogen is inseparable, both of which are vital female hormones. The role of estrogen is mainly to promote the development and maturity of female secondary sexual characteristics. In contrast, progesterone further promotes the growth and maturity of secondary sexual characteristics based on the role of estrogen, and there is a synergistic effect between the two
Estrogen and Progesterone
Estrogen, also known as the female hormone, is a primarily female hormone. It promotes the maturation of female genital organs and the appearance of secondary sexual characteristics and maintains normal libido and reproductive function hormones. There are two main groups (both steroid hormones), estrogen and progesterone.
Estrogen is secreted by the follicular cells of the ovary (the placenta also secretes estrogen), mainly estradiol and inactivated in the liver, converted to estriol and estrone, and combined with glucuronic acid and excreted in the urine. During pregnancy, the placenta secretes large amounts of estriol.
Estrogen is the sex hormone of female vertebrates and promotes the appearance of secondary sexual characteristics. Mammals can also make the filtrate after ovulation into the corpus luteum. They can secrete progesterone (also known as progesterone hormone, progesterone) called the second estrogen, which can control pregnancy lactation, and further promote secondary sexual characteristics mature function.